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S. aureus JE2 biofilms were established after 24h incubation in LB 50mM NaB 0.5% w/v glucose. These biofilms were treated with Proteinase K (0.1mg/mL) or DNase I (140U/mL) for 16h. Following treatment, biofilm formation was quantified by measured crystal violet staining. Data represent the mean ± standard error of the mean of three independent biological replicates. P-values are from a 2-way ANOVA test with Tukey correction for multiple comparisons.

Journal: bioRxiv

Article Title: Butyrate synergizes with glucose to promote anaerobic growth of Staphylococcus aureus via anaplerotic metabolism and stress response pathways

doi: 10.64898/2026.04.07.717036

Figure Lengend Snippet: S. aureus JE2 biofilms were established after 24h incubation in LB 50mM NaB 0.5% w/v glucose. These biofilms were treated with Proteinase K (0.1mg/mL) or DNase I (140U/mL) for 16h. Following treatment, biofilm formation was quantified by measured crystal violet staining. Data represent the mean ± standard error of the mean of three independent biological replicates. P-values are from a 2-way ANOVA test with Tukey correction for multiple comparisons.

Article Snippet: RNA was purified using the Zymo RNA Clean & Concentrator-5 kit (Zymo) with on-column DNase I treatment (5 μL DNase I and 35 μL DNA digestion buffer, 30 min, room temperature).

Techniques: Incubation, Staining